Journal
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
Volume 154, Issue 1, Pages 29-36Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpa.2009.01.004
Keywords
Biogenic amine; Litopenaeus vannamei; mRNA; Na+/K+-ATPase; Shrimp; Vacuolar-type H/-ATPase
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Funding
- Scientific Research Foundation for Outstanding Young Scientist of Shandong Province [2006BS07005]
- Program for New Century Excellent Talents in University [NCET-06-0597]
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We used semi-quantitative reverse transcription and the polymerase chain reaction (RT-PCR) to identify and characterize the mRNA of Na+/K+-ATPase alpha-subunit and Vacuolar-type H/-ATPase (V-ATPase) beta-subunit prepared from gills of the white shrimp Litopenaeus vannamei. Western blot and immunocytochemistry analysis were employeed to investigate the effects of biogenic amines such as dopamine (DA) and 5-hydroxytryptamine (5-HT) injection on expression of protein abundance and immunostained sites. Our results show that the gill Na+/K+-ATPase of 5-HT treatments increased lag to those of DA treatments. However, the DA and 5-HT have no significant effects on the activity of V-ATPase. The biogenic amines could induce the synthesis of Na+/K+-ATPase alpha-subunit mRNA in the first 12 h. The degree of change in Na+/K+-ATPase alpha-subunit mRNA expression were different between DA and 5-HT treated groups which depend on the dose. The expression of V-ATPase beta-subunit mRNA was not markedly affected by the biogenic amines. Concomitant elevation of Na+/K+-ATPase activity, alpha-subunit mRNA as well as protein abundance in response to injected biogenic amines was also found in Na+/K+-ATPase immunostained sites within the gill epithelium (C) 2009 Published by Elsevier Inc.
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