Journal
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
Volume 151, Issue 2, Pages 151-158Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpa.2008.06.012
Keywords
mitochondria-rich cell; Na+/K+-ATPase; Na+/H+ exchanger-3; Na+/Cl- cotransporter; Na+/K+/2Cl(-) cotransporter-1a; tilapia; Oreochromis mossambicus
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We explored molecular and morphological alteration in gill mitochondria-rich (MR) cells of Mozambique tilapia, Oreochromis mossambicus, acclimated to deionized freshwater (DFW), freshwater (FW), 1/3-diluted seawater (1/3 SW) and seawater (SW). Scanning electron microscopic observations revealed that the apical membrane of MR cells appeared as a flat or slightly projecting disk in DFW and FW, being larger in DFW than in FW. In contrast, the apical membrane typically formed a pit structure in 1/3 SW and SW. The. mRNA expression levels of Na+/H+ exchanger-3 (NHE3) and Na+/Cl- cotransporter (NCC) in the gills were increased with decreasing environmental salinity, whereas Na+/K+/2Cl(-) cotransporter-1a (NKCC1a) expression was upregulated by increasing salinity. Immunofluorescence staining showed that the MR cell population of DFW- and FW-acclimated tilapia consisted mostly of MR cells with apical NHE3 and those with apical-NCC; MR cells with basolateral NKCC1a dominated in SW-acclimated tilapia. These results indicated that apical-NHE3 and apical-NCC MR cells were ion-absorbing cells, and that basolateral-NKCC1a MR cells were ion-secreting cells. In fish acclimated to 1/3 SW, both ion-absorbing and secreting cells existed in the gills, suggesting that fish in near-isotonic water were equipped with mechanisms of both hyper- and hypoosmoregulation to prepare for environmental salinity changes. (C) 2008 Elsevier Inc. All rights reserved.
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