Journal
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
Volume 149, Issue 3, Pages 255-266Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cbpa.2007.12.005
Keywords
Mycobacterium tuberculosis; E. coli; M. bovis; sulfate transport; SLC26A6; SnlP
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Funding
- NIDDK NIH HHS [DK34854, T32 DK007199, R01 DK43495, P30 DK034854, R37 DK043495-17, R37 DK043495, T32 DK07199, P30 DK034854-15S19007] Funding Source: Medline
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Growth and virulence of mycobacteria requires sulfur uptake. The Mycobacterium tuberculosis genome contains, in addition to the ABC sulfate permease cysTWA, three SLC26-related SulP genes of unknown function. We report that induction of Rv1739c expression in E. coli increased bacterial uptake of sulfate, but not Cl-, formate, or oxalate. Uptake was time-dependent, maximal at pH 6.0, and exhibited a K-1/2 for sulfate of 4.0 mu M. Na+-independent sulfate uptake was not reduced by bicarbonate, nitrate, or phosphate, but was inhibited by sulfite, selenate, thiosulfate, N-ethylmaleimide and carbonyl cyanide 3-chloro-phenylhydrazone. Sulfate uptake was also increased by overexpression of the Rv1739c transmembrane domain, but not of the cytoplasmic C-terminal STAS domain. Mutation to serine of the three cysteine residues of Rv1739c did not affect magnitude, pH-dependence, or pharmacology of sulfate uptake. Expression of Rv1739c in a M. bovis BCG strain lacking the ABC sulfate permease subunit CysA could not complement sulfate auxotrophy. Moreover, inducible expression of Rv1739c in an E. coli strain lacking CysA did not increase sulfate uptake by intact cells. Our data show that facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, and suggest that Rv1739c may be a CysTWA-dependent sulfate transporter. (C) 2007 Elsevier Inc. All rights reserved.
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