Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 172, Issue -, Pages 407-413Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2018.08.064
Keywords
Signal amplification; Hemin/G-quadruplex; Y-shaped DNA; Platelet-derived growth factor-BB
Funding
- National Natural Science Foundation of China [21475115]
- Program for University Innovative Research Team of Henan [15IRTSTHN001]
- Henan Provincial Science and technology innovation team [C20150026]
- Nanhu Scholars Program of XYNU
- Nanhu Scholars Program for Young Scholars of XYNU
- Natural Science Foundation of Henan Province [162300410230]
- Henan Science and Technology Cooperation Project [172106000064]
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A highly sensitive electrochemical biosensor for detection of platelet-derived growth factor-BB (PDGF-BB) is developed by using Se-doped multi-walled carbon nanotubes (MWCNTs)-graphene hybrids as electrode supporting substrate, hemin/G-quadruplex as trace labels and Y-shaped DNA-aided target recycling as signal magnifier. The aptamer-containing hairpin probes were first immobilized on the electrode. When target PDGF-BB was added, the aptamer binded PDGF-BB to trigger catalytic assembly of two other hairpins to form many G-quadruplex Y-junction DNA structures, which released PDGF-BB to again bind the intact aptamer to initiate another assembly cycle. G-quadruplex/hemin complexes were produced when hemin was added to generate substantially amplified current output. The developed assay showed a linear range toward PDGF-BB from 0.1 pM to 10 nM with a detection limit of 27 fM (S/N = 3). The method showed excellent specificity and repeatability, and could be expediently applied for sensitive detection of other molecules by simply changing the aptamers.
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