Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 116, Issue -, Pages 211-218Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2014.01.003
Keywords
Surface plasmon resonance; Graphene oxide; Au bipyramids,IgM
Funding
- National Natural Science Foundation of China [20727003, 21075049, 21105037]
- Program for New Century Excellent Talents in University [NECT-10-0443]
- Special-funded Programme on National Key Scientific Instruments and Equipment Development [2012YQ090194]
- Science and Technology Developing Foundation of Jilin Province [20100356, 20110162]
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A sensitive and selective wavelength-modulation surface plasmon resonance (SPR) biosensor based on graphene oxide (GO) and Au bipyramids (AuBPs) is reported for determination of bovine IgM. GO sheets with lengths of 100-300 nm are synthesized and assembled on amine-modified Au film. The large surface area and abundant functional groups of GO allow the efficient immobilization of antibody. AuBPs are nanoparticles with a penta-twinned crystal structure, which have a sharp localized surface plasmon resonance (LSPR) band because of their high monodispersity. In the optimal conditions, the GO-based biosensor with AuBPs as sensitivity enhancers shows a satisfactory response to bovine IgM in the concentration range of 0.03-32 mu g mL(-1). For contrast, traditional biosensor, GO-based biosensor and GO-based biosensor with Au nanorods (AuNRs) as sensitivity enhancers for antigen detection were also investigated. Consequently, the as-prepared GO sheets function as promising support for antibody and GO-based SPR biosensor using AuBPs as enhancers has the highest sensitivity among the four types of biosensors. (C) 2014 Elsevier B.V. All rights reserved.
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