Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 117, Issue -, Pages 68-74Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2014.01.041
Keywords
Adsorption; DNA; Escherichia coli; Electrochemistry; Surface Plasmon Resonance
Funding
- National Natural Science Foundation of China [81170991, 21077081, 20921062]
- Fundamental Research Funds for Central Universities [1103005]
Ask authors/readers for more resources
In this work, we proposed an immobilization scheme targeting Escherichia coli bacterial cells onto gold surface utilizing polypeptide RGD's binding specificity to gold particles. Adsorption kinetics of extracellular Herring Sperm DNA on E. coli was then studied using electrochemical methods along with surface plasmon resonance spectroscopy through this immobilization scheme. The adsorption equilibrium constants of DNA adsorbing to E. coli from electrochemical method and surface plasmon resonance spectroscopy, were (5.596 +/- 0.462) x 105 Lmol(-1) and (1.24 +/- 0.361) x 105 Lmol(-1), accordingly. Importantly, this is the first study that used an electrochemical method to express the adsorptive action of DNA by E. coli. (C) 2014 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available