Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 116, Issue -, Pages 378-382Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2013.07.022
Keywords
Covalent immobilization; Horseradish peroxidase; PM-IRRAS; Bioactivity; Biofunctionalization
Funding
- German Federal Ministry of Education and Research (BMBF) [13N9788]
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A new coupling strategy for biomacromolecules with (3-mercaptopropyl)trimethoxysilane (3MPTMS) and 11-(triethoxysilyl)undecanal (TESU) on gold surfaces is. This immobilization protocol was utilized for the enzyme horseradish peroxidase (HRP). To study the reactions and resulting structures, PM-IRRAS measurements were performed. PM-IRRAS shows there is structure preservation of the HRP when the new coupling strategy is used in contrast to non-specific adsorption on gold. The biological activity of adsorbed and immobilized HRP was measured by the enzyme catalyzed oxidation of 3,5,3',5'-tetramethylbenzidine. Covalent immobilization of HRP on TESU film compared to physisorption of HRP shows higher enzyme activity on gold surfaces, confirming the structural preservation detected by PM-IRRAS. (C) 2013 Elsevier B.V. All rights reserved.
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