Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 112, Issue -, Pages 108-112Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2013.07.045
Keywords
Polyethylenimine; Barium titanate; Transfection; Second harmonic generation
Funding
- Nanobiology Interdisciplinary Graduate Training Program of the W. M. Keck Center for Interdisciplinary Bioscience Training of the Gulf Coast Consortia (NIH) [T32 EB009379]
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Barium titanate nanoparticles (BT NP) belong to a class of second harmonic generating (SHG) nanoprobes that have recently demonstrated promise in biological imaging. Unfortunately, BT NPs display low cellular uptake efficiencies, which may be a problem if cellular internalization is desired or required for a particular application. To overcome this issue, while concomitantly developing a particle platform that can also deliver nucleic acids into cells, we coated the BT NPs with the cationic polymer polyethylenimine (PEI)-one of the most effective nonviral gene delivery agents. Coating of BT with PEI yielded complexes with positive zeta potentials and resulted in an 8-fold increase in cellular uptake of the BT NPs. Importantly, we were able to achieve high levels of gene delivery with the BT-PEI/DNA complexes, supporting further efforts to generate BT platforms for coupled imaging and gene therapy. (C) 2013 Elsevier B.V. All rights reserved.
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