Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 110, Issue -, Pages 8-14Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2013.04.025
Keywords
Graphene/CdS; Nanocomposite; DNA; Electrochemical biosensor; Phenformin
Funding
- National Natural Science Foundation of China [61172005]
- National High Technology Research and Development Program of China (863 Program) [2012AA06A304]
- Opening Foundation of State Key Laboratory of Chemo/Biosensing and Chemometrics [200908]
- Fundamental Research Funds for the Central Universities [2011TS062]
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Graphene/cadmium sulphide (GR-CdS) nanocomposite was synthesized via a low temperature process in aqueous solution. The as-prepared nanocomposite was characterized by scanning electron microscopy, UV-visible spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction. The impedance analysis indicated that GR-CdS nanocomposite possessed outstanding electrochemical performance for facile electron transfer. When DNA was immobilized on GR-CdS (DNA/GR-CdS) modified electrode, the electrochemical oxidation of guanine and adenine in DNA residue bases was significantly promoted. Due to the interaction of DNA with phenformin, the voltammetric current of guanine or adenine on the DNA/GR-CdS electrode was decreased when phenformin was present in the electrolytic solution. Under optimized conditions, the signal of guanine on DNA/GR-CdS electrode decreased linearly with increasing the concentration of phenformin in the range of 1.0 x 10(-6) mol L-1 to 1.0 x 10(-3) mol L-1. The proposed DNA-based electrochemical biosensor was successfully applied to the determination of phenformin in real samples. (c) 2013 B.V. All rights reserved.
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