Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 106, Issue -, Pages 46-50Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2013.01.031
Keywords
Alpha-amylase; Polyaniline; Immobilization; Stability
Funding
- University Grants Commission of India
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Alpha-amylase was immobilized onto polyaniline-polyvinyl alcohol (PAni-PVA) film by cross linking with glutaraldehyde. The activity yield and immobilization efficiency was calculated as 13.58% and 30.60% respectively. The PAni-PVA-Amylase films were characterized by Fourier Transform Infrared Spectroscopy (FTIR) and amylase activity assay. The immobilized a-amylase had its optimum activity at pH 7.0 and 25 degrees C. The immobilization of alpha-amylase onto the PAni-PVA films protected the enzyme from Fe2+ inhibition. The PAni-PVA-Amylase films could be used effectively for 30 cycles with 60% retention of the activity. (C) 2013 Elsevier B.V. All rights reserved.
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