Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 107, Issue -, Pages 152-159Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2013.01.071
Keywords
Zwitterionic polymer; Sulfobetaine; Carboxybetaine; Non-fouling; Polyelectrolyte multilayer
Funding
- National Science Council, Taiwan [100-2221-E-002-114-MY2, NSC-99-2113-M-027-001-MY3]
Ask authors/readers for more resources
Non-fouling surfaces that resist non-specific protein adsorption and cell adhesion are desired for many biomedical applications such as blood-contact devices and biosensors. Therefore, surface conjugation of anti-fouling molecules has been the focus of many studies. In this study, layer-by-layer polyelectrolyte deposition was applied to create an amine-rich platform for conjugation of zwitterionic polymers. A trilayer polyelectrolyte (TLP) coating representing poly(ethylene imine) (PEI), poly(acrylic acid)-g-azide and PEI was deposited on various polymeric substrates via layer-by-layer deposition and then crosslinked via UV irradiation. Carboxyl-terminated poly(sulfobetaine methacrylate) p(SBMA) or poly(carboxybetaine methacrylate) p(CBMA) was then conjugated onto TLP coated substrates via a carbodiimide reaction. Our results demonstrate that the zwitterionic polymers could be easily conjugated over a wide pH range except under alkaline conditions, and almost completely block protein adsorption and the attachment of L929 cells and platelets. Therefore, this method has outstanding potential in biomedical applications that require low-fouling surfaces. (C) 2013 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available