Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 103, Issue -, Pages 566-571Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2012.11.010
Keywords
Direct electron transfer; Glucose oxidase; Glucose biosensor; Overoxidized polypyrrole; Au nanoparticles
Funding
- Institute for Advanced Studies in Basic Science (IASBS) [G2102IASBS119]
- Iran National Science Foundation (INSF) [90003581]
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An overoxidized polypyrrole (OOPPy) film was electrodeposited on a glassy carbon electrode (GCE) and the modified electrode (GCE/OOPPy) was then decorated with Au nanoparticles (nanoAu). Glucose oxidase was immobilized on the surface of nanoAu decorated OOPPy modified GCE to fabricate a novel glucose biosensor (GCE/OOPPy-nanoAu/GOx). Cyclic voltammetry, electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM) were used to characterize the modified electrodes. A pair of well-defined redox peaks with a formal potential (E-o') of -0.449 V and a peak to peak separation (Delta E-p) of 28 mV was observed for the direct electron transfer (DET) of the immobilized GOx. The electron transfer rate constant (k(s)) was calculated to be 10.3 s(-1). The fabricated glucose biosensor was employed for the determination of glucose in the concentration range between I and 8 mM using cyclic voltammetry and amperometry. The results clearly demonstrate that nanoAu decorated OOPPy film is an excellent biocompatible scaffold for the immobilization of GOx and fabrication of a glucose biosensor. (C) 2012 Elsevier B.V. All rights reserved.
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