Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 112, Issue -, Pages 42-50Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2013.07.037
Keywords
Fibril; D-periodicity; Banding pattern; Surface charge; Electrostatic interactions; Salt bridge
Funding
- National Science Foundation [BES-0404000]
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Even though the behavior of collagen monomers self-assembling into fibrils is commonly understood in terms of hydrophobic and electrostatic interactions, the mechanisms that drive their ordered, longitudinal alignment to form a characteristic periodicity are still unclear. By introducing various salts into the collagen fibrillogenesis system, the intermolecular interactions of fibril formation were studied. We found that the pH and ion species play a critical role in forming native fibrils. Turbidity and electron microscopy revealed that collagen self-assembled into fibrils with a native banding pattern in the presence of multivalent ions. The isoelectric point of collagen in 12 mM of NaCl is pH 8.9; it shifted to pH 9.4 and pH 7.0 after adding 10 mM CaCl2 and Na2SO4, respectively. Native fibrils were reconstituted at pH 7.4 in salts with divalent anions and at pH 9.0 in salts with divalent cations. Circular dichroism spectroscopy showed a loss of helicity in the conditions where fibrillogenesis was unable to be achieved. The multivalent ions not only change the surface charge of collagen, but also facilitate the formation of fibrils with the native D-periodic banding pattern. It is likely that the binding multivalent ions induce the like-charge attraction and facilitate monomers' longitudinal registration to form fibrils with the native banding. Published by Elsevier B.V.
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