Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 94, Issue -, Pages 274-280Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2012.02.001
Keywords
Nanoparticles; 17-AAG; Folic acid; Breast cancer; Polylactide-co-glycolide
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Low water solubility and hepatotoxicity limited the clinical use of 17-allylamino-17-demethoxy geldanamycin (17-AAG), an inhibitor of heat shock protein 90 (HSP90). Folate targeted polylactide-co-glycolide-polyethylene glycol-folic acid (PLGA-PEG-FA) nanoparticles containing 17-AAG were prepared and characterized. Cellular uptake and in vitro cytotoxicity of the prepared nanoparticles were determined in MCF-7 human breast cancer cells. The particle size of 17-AAG loaded folate targeted nanoparticles was 238.67 +/- 3.52 nm, drug loading was 8.25 +/- 2.49% and about 80% of drug was released from the nanoparticles over 10 days. Cellular uptake studies showed much higher intracellular uptake of folate targeted nanoparticle as compared to nontargeted nanoparticles. Cytotoxicity study showed 2 fold increase (P < 0.05, n = 3) in the cytotoxicity of folate targeted nanoparticle in comparison to free drug or nontargeted nanoparticles. Due to their targeting ability, nanometer size, high drug loading and controlled release behavior, 17-AAG loaded PLGA-PEG-FA nanoparticles might be developed as a targeted delivery system for breast and other cancer treatment. (C) 2012 Elsevier B.V. All rights reserved.
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