Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 92, Issue -, Pages 175-179Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2011.11.040
Keywords
Chitosan; Cross-linking; Factorial design; Insulin; Microspheres; Taguchi design
Funding
- Fundacao para a Ciencia e Tecnologia do Ministerio da Ciencia e Tecnologia [PTDC/SAU-FAR/113100/2009]
- Fundação para a Ciência e a Tecnologia [PTDC/SAU-FAR/113100/2009] Funding Source: FCT
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Insulin-loaded chitosan microspheres were engineered by emulsion cross-linking method using glutaraldehyde as cross-linker. Taguchi orthogonal method was applied to optimize the production time and reduce the number of experiments required to obtain an optimized formulation. Three variables were evaluated, i.e. chitosan and glutaraldehyde concentrations, and cross-linking time at three levels. The dependent variables were the mean particle size and the encapsulation efficiency. The optimal formulation was obtained with chitosan 3% (w/v), glutaraldehyde 3.5% (v/v), and cross-linking time of 5 h, characterized by microspheres with a mean particle size of 29.5 mu m, and insulin encapsulation efficiency of 71.6 +/- 1.3%. In vivo studies were carried out using male Wistar albino rats, revealing a significant reduction in blood glucose level after administration of the optimized formulation, in comparison to a subcutaneous insulin injection. Chitosan microspheres were superior in terms of sustaining protein release over conventional insulin therapy. (C) 2011 Elsevier B.V. All rights reserved.
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