4.7 Article

Immobilization of bovine serum albumin onto porous polyethylene membranes using strongly attached polydopamine as a spacer

Journal

COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 86, Issue 1, Pages 111-118

Publisher

ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2011.03.027

Keywords

Polyethylene; Porous membranes; Surface modification; Dopamine; Bovine serum albumin

Funding

  1. National Nature Science Foundation of China [50803054]
  2. China Postdoctoral Science Foundation [200902633]
  3. Zhejiang Provincial Nature Science Foundation of China [Y4100204]
  4. 973 Program of China [2009CB623402]
  5. Fundamental Research Funds for the Central Universities (MOE Engineering Research Center of Membrane and Water Treatment Technology, Zhejiang University)

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Based on the self-polymerization and strong adhesion characteristics of dopamine in aqueous solution, a novel and convenient approach was developed to immobilize protein onto porous polyethylene (PE) membranes. A thin polydopamine (pDA) layer was formed and tightly coated onto PE membrane by dipping simply the membrane into dopamine aqueous solution for a period of time. Subsequently, bovine serum albumin (BSA) was bound onto the obtained PE/pDA composite membranes via the coupling between BSA and the reactive polydopamine layer. The firm immobilization of polydopamine layer and BSA was verified by attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and X-ray photoelectron spectroscopy (XPS). The results of water contact angle measurement showed that the hydrophilicity of PE membrane was significantly improved after coating polydopamine and binding BSA. The experiments of blood platelet adhesion indicated that BSA-immobilized PE membrane had better blood compatibility than the unmodified PE and the PE/pDA composite membranes. The investigations on hepatocyte cultures and cell viability revealed that the polydopamine coating endowed PE membrane with significantly improved cell compatibility. Compared to BSA surface, polydopamine surface is more favorable for cell adhesion, growth, and proliferation. (C) 2011 Elsevier B.V. All rights reserved.

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