Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 87, Issue 2, Pages 289-292Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2011.05.031
Keywords
Quartz crystal microbalance; Hg2+ ion; Gold nanoparticle; Thymine
Funding
- Chinese Academy of Sciences [KJCX2-YW-H18]
- National Sci-Tech Major Special Item for Water Pollution Control and Management [2009ZX075287-007-03]
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A simple and sensitive method was developed for the detection of mercury ions with quartz crystal microbalance (QCM), based on the specific thymine-Hg2(+)-thymine (T-Hg2+-T) interaction and gold nanoparticle-mediated signal amplification. To enhance the sensitivity of detection a sandwich hybridization approach was adopted in this work. The QCM gold surface was modified with the probe SH-oligonucleotides (Oligo-1) and 6-Mercapto-1-hexanol to form an active surface for the hybridization of a longer ss-DNA (Oligo-2), and then Oligo-3 hybridazated with an excess and matching part of Oligo-2. In all oligonucleotides, there existed T bases. In the presence of Hg2+ ions, special T-Hg2+-T reaction greatly enhanced the hybridization of oligonucleotides and detection sensitivity. The gold nanoparticle (Au NPs) amplifier method further increased the sensitivity of detection. A detection sensitivity of 5 nM Hg2+ was obtained in the QCM system, whereas other coexisting metal ions (such as Ni2+, Mg2+, Co2+, Cr3+, Pb2+, Cd2+, Mn2+, Ba2+) had no significant interference. This method reveals a new approach for the manufacture of a kind of simple and low cost sensors for the Hg2+ detection. (C) 2011 Elsevier B.V. All rights reserved.
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