Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 83, Issue 2, Pages 367-375Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2010.12.011
Keywords
Poly(lactic-co-glycolic acid); Multiwalled carbon nanotube; Mesenchymal stem cells; Nanocomposites
Funding
- Science and Technology Innovation Project of Fujian Province for Young Scientific Researchers, China [2008F3097]
- National Natural Science Foundation of China [90923042, 30901175]
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Biodegradable poly(lactic-co-glycolic acid) (PLGA)/carboxyl-functionalized multi-walled carbon nanotube (c-MWCNT) nanocomposites were successfully prepared via solvent casting technique. Rat bone marrow-derived mesenchymal stem cells (MSCs) were employed to assess the biocompatibility of the nanocomposites in vitro. Scanning electron microscopy (SEM) observations revealed that c-MWCNTs gave a better dispersion than unmodified MWCNTs in the PLGA matrix. Surface properties were determined by means of static contact angle, X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) analysis. The presence of c-MWCNTs increased the mechanical properties of the nanocomposites. Seven-week period in vitro degradation test showed the addition of c-MWCNTs accelerated the hydrolytic degradation of PLGA. In addition, SEM proved that the cells could adhere to and spread on films via cytoplasmic processes. Compared with control groups. MSCs cultured onto PLGA/c-MWCNT nanocomposites exhibited better adhesion and viability and also displayed significantly higher production levels of alkaline phosphatase (ALP) over 21 days culture. These results demonstrated that c-MWCNTs modified PLGA films were beneficial for promoting cell growth and inducing MSCs to differentiate into osteoblasts. This work presented here had potential applications in the development of 3-D scaffolds for bone tissue engineering. (C) 2010 Elsevier B.V. All rights reserved.
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