Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 84, Issue 1, Pages 26-34Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2010.12.005
Keywords
Electrospinnning; Nanofibrous scaffolds; Tissue engineering
Funding
- Natural Science Foundation of China [51073005, 81071260]
- Beijing Natural Science Foundation [KZ201010012012]
- PHR (IHLB)
- 863 Project [2007AA021906]
- 973 Project [2010CB933501]
- Tianjin Science Technology Research Funds of China [10JCZDJC17000]
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Nanostructured biocomposite scaffolds of poly(L-lactide) (PLLA) blended with collagen (coll) or hydroxyapatite (HA), or both for tissue engineering application, were fabricated by electrospinning. The electrospun scaffolds were characterized for the morphology, chemical and tensile properties by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), water contact angle (WCA), Fourier transform infrared (FUR) measurement, and tensile testing. Electrospun biocomposite scaffolds of PLLA and collagen or (and) HA in the diameter range of 200-700 nm mimic the nanoscale structure of the extracellular matrix (ECM) with a well-interconnection pore network structure. The presence of collagen in the scaffolds increased their hydrophility, and enhanced cell attachment and proliferation, while HA improved the tensile properties of the scaffolds. The biocompatibility of the electrospun scaffolds and the viability of contacting cells were evaluated by 4',6-diamidino-2-phenylindole dihydrochloride (DAPI) nuclear staining and by fluorescein diacetate (FDA) and propidium iodide (PI) double staining methods. The results support the conclusion that 293T cells grew well on composite scaffolds. Compared with pure PLLA scaffolds a greater density of viable cells was seen on the composites, especially the PLLA/HA/collagen scaffolds. (C) 2010 Elsevier B.V. All rights reserved.
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