Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 85, Issue 1, Pages 12-18Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2010.09.028
Keywords
Gradient surface; ATRP; Polymer films; Cell culture; Adhesion
Funding
- Nature Science Foundation of China [20934003, 20774084]
- Major State Basic Research Program of China [2005CB623902]
- Key Laboratory for Supramolecular Structure and Materials [SKLSSM200702]
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Using surface initiated atomic transfer radical polymerization (ATRP) and an injection method, a poly(N-isopropylacrylamide)-b-poly(acrylic acid)-g-RGD (PNIPAAm-b-PAA-g-RGD) gradient surface was prepared. First, a thermoresponsive surface with a constant thickness of PNIPAAm was fabricated, onto which the AA monomers were block copolymerized using the PNIPAAm macromolecules as initiators. During this process, a continuous injection method was employed to yield a molecular weight gradient of PAA on the underlying uniform PNIPAAm layer. RGD peptide was finally covalently immobilized onto the PAA gradient by carbodiimide chemistry. In vitro culture of HepG2 cells showed that immobilization of the RGD peptide could accelerate cell attachment, while the thermoresponsive layer beneath could effectively release the cells by simply lowering temperature. Thus, the PNIPAAm-b-PAA-g-RGD gradient surface, combining the thermal response with cell affinity properties, can well regulate the cell adhesion and detachment, which may thus be useful for investigation of cell-substrate interactions with a smaller number of samples. (C) 2010 Elsevier B.V. All rights reserved.
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