Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 86, Issue 1, Pages 212-217Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2011.04.001
Keywords
DNA; Lipid bilayer; Divalent cations; Fluorescence spectroscopy; UV-vis spectrophotometry
Funding
- JINR [07-4-1069-09/2011]
- VEGA [1/0292/09]
Ask authors/readers for more resources
We studied the effect of divalent alkaline earth metal cations Ca(2+). Mg(2+) and transition metals Co(2+), Ni(2+), Cu(2+) and Zn(2+) on DNA condensation and its protection against thermal denaturation in presence of dioleoylphosphatidylcholine liposomes (DOPC). Experimental results have shown that Ca(2+). and Mg(2+) as well as Zn(2+) mediate DNA condensation. Cu(2+) causes DNA double helix destabilization, and does not mediate binding between DNA and DOPC liposomes. Co(2+) and Ni(2+) can interact with DNA on both ways mentioned above. Static light scattering was use to follow the size of aggregates in DNA condensation process. Phospholipid bilayer and divalent cations protect condensed DNA against thermal destabilization. The highest stabilization effect was found in aggregates with Ca(2+) and Zn(2+). whereas in presence of either Co(2+) or Ni(2+) some volume fraction of DNA is denatured. (C) 2011 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available