Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 86, Issue 1, Pages 225-231Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2011.04.005
Keywords
Blood-brain barrier; Human brain-microvascular endothelial cells; Human astrocyte; Transendothelial electrical resistance; Permeability
Funding
- National Science Council of the Republic of China
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A blood-brain barrier (BBB) model in vitro was established by cultivating human brain-microvascular endothelial cells (HBMECs) with the regulation of human astrocytes (HAs) (HBMEC/HA). Astrocyte-conditioned medium (ACM) was employed to constitute a confluent monolayer of HBMECs without directly conjugated HAs. HBMECs exhibited an orientated multiplication on the supporting membrane; while HAs grew in an overlapping fashion. In addition, HBMECs could propagate over the membrane pore, and the end-feet of HAs extended into the membrane pore to improve the integral feature of the BBB. HBMEC/HA demonstrated a high transendothelial electrical resistance (TEER) about 230 Omega cm(2) and low permeability of propidium iodide (PI) about 4 x 10(-6) cm/s. The order in TEER was HBMEC/HA > HBMECs with 100% ACM > HBMECs with 50% ACM > HBMECs. The reverse order was valid for the permeability of PI and uptake of calcein-AM by HBMECs. The tranwell culture of HBMECs and HAs displays appropriate characteristics of the BBB and can be applied to estimate the delivery efficiency of therapeutic chemicals for the brain-related disease. (C) 2011 Elsevier B.V. All rights reserved.
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