Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 86, Issue 2, Pages 384-389Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2011.04.026
Keywords
Biosurfactant; Surface tension; Serratia marcescens; Heavy oil; Box-Behnken
Funding
- Instituto Mexicano del Petroleo [IMP-D.00417]
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The strain SmSA, identified as Serratia marcescens and known as a biosurfactant producer, was isolated from hydrocarbon contaminated soil from Veracruz, Mexico. The interactions among the C/N, C/Mg and C/Fe ratios have not been examined for this microorganism. In this work was evaluated the effect of these nutrients at three levels using a mineral medium with glucose as the carbon source. A Box-Behnken experimental design was utilised to maximise biosurfactant production, which was assessed by oil spreading and surface tension tests. The treatment with C/N = 5, C/Fe = 26,000 and C/Mg = 30 showed the best result since the surface tension was reduced to 30 mN m(-1). The multiple regression and response surface analyses indicated that the interaction between C/N and C/Mg had the utmost effect on the reduction of surface tension and biosurfactant production. The conditions of the best treatment were used to scale up biosurfactant production in a 3 L bioreactor giving a yield of 4.1 g L-1 of pure biosurfactant. It was found that the biosurfactant was mainly produced in the exponential phase and decreased the surface tension to 31 mN m(-1). The contact between the biosurfactant with heavy oil (15 degrees API) increased its displacement from 9.3 to 18 cm. (C) 2011 Elsevier B.V. All rights reserved.
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