Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 80, Issue 2, Pages 180-183Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2010.06.001
Keywords
Silver; Nanoparticles; IgG; Immunoagglutination; Antigen detection
Funding
- State grant of Republic of Armenia [445.515]
- BIO-VAR Ltd.
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Immunoagglutination properties of silver nanoparticles sensitized with antibodies to human IgG for determination of IgG were studied. Effects of various pH on sensitization and consequent immunoagglutination process were investigated. It was shown that the best results were obtained when sensitization and immunoagglutination were conducted at isoelectric point of IgG molecule. It was demonstrated that immunoassay with colloidal silver nanoparticles provides the detection of antigen in wide range of concentrations. (C) 2010 Elsevier B.V. All rights reserved.
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