Effect of edelfosine on tumor and normal cells model membranes-A comparative study

This work is aimed at comparing the effect of edelfosine (Ed) on normal and tumor cell membrane imitated by the Langmuir monolayers prepared from cholesterol and phosphatidylcholines. To keep the differences in the fluidity of these membranes, model membrane of normal cell has been composed from Saturated phospholipid (DPPC) and contained a higher proportion of cholesterol (cholesterol:DPPC = 0.67) than tumor cell model membranes prepared from Unsaturated phosphatidylcholine (cholesterol:POPC = 0.25). The results proved that the incorporation of edelfosine modifies the organization and interactions between molecules in both model systems. The interactions in cholesterol/DPPC/edelfosine monolayers are stronger than in cholesterol/DPPC film. On the other hand, the interactions in cholesterol/POPC/edelfosine system are weaker than in cholesterol/POPC monolayer, thus the incorporation of edelfosine is, from thermodynamical point of view, unfavorable for binary cholesterol/POPC monolayer. Edelfosine has been found to increase the fluidity of model membranes, however, at a lower concentration (up to 5% of edelfosine in model system) this compound affects only the condensation Of tumor cell model membrane, while practically does not modify the organization of normal model system. It has been suggested that edelfosine inserts into tumor cellular membranes more easily than into normal cell membrane and cholesterol/edelfosine interactions determine the interactions between molecules in the investigated mixed monolayers. (C) 2009 Elsevier B.V. All rights reserved.