Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 74, Issue 1, Pages 266-273Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2009.07.029
Keywords
Brevibacterium cosei; Polyhydroxybutyrate; Green chemistry; CCRD; PHB nanoparticles
Funding
- Department of Biotechnology, Ministry of Science and Technology, Government of India, New Delhi [BT/PR-10211/BCE/08/615/2007]
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Polyhydroxyalkanoates (PHAs) are natural, biodegradable polymers accumulated by bacteria under nutritional exhausted condition where carbon source is in excess. A gram positive bacterium (designated strain SRKP2) that potentially accumulated polyhydroxybutyrate (PHB) was isolated from dairy industrial waste. From its morphological and physiological properties and nucleotide sequence of its 16S rRNA, it was suggested that strain SRKP2 was similar to Brevibacterium casei. PHAs were synthesized from a medium containing dairy waste, yeast extract and sea water. The synthesized PHAs were characterized by FT-IR as Polyhydroxybutyrate (PHB). Response surface methodology was applied to optimize the production of PHB. From the optimized medium the yield of PHB was found to be 2.940 g/L. Here we report the direct use of dairy waste and sea water as potential sources for the production of PHB. Produced PHB was used to synthesize nanoparticles using solvent displacement technique. (c) 2009 Elsevier B.V. All rights reserved.
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