Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 74, Issue 1, Pages 298-303Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2009.07.036
Keywords
Layer-by-layer; Chitosan-DNA complex; Hyaluronic acid; Cytocompatibility; Surface-mediated gene delivery
Funding
- Natural Science Foundation of China [NSFC-20774082, 50830106]
- 863 National High-Tech RD Program [2006AA03Z329, 2006AA03Z444]
- Open Project of State Key Laboratory of Supramolecular Structure and Materials [SKLSSM200911]
- Program for New Century Excellent Talents in University [NCET-05-0527]
- Science and Technology Projects of Zhejiang Province [2007C24008]
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Sustained release of DNA from the surface of materials represents a promising approach to combine the gene therapy and implantable biomaterials. The nonviral chitosan-DNA complexes were incorporated into the multilayer via layer-by-layer deposition with hyaluronic acid (HA). The UV-vis spectroscopy and atomic force microscopy (AFM) results showed the successful construction of the nonviral complex contained multilayers. The complexes were releasable in physiological condition and a sustained release manner was gained when the multilayer was crosslinked. The cell viability test and the gene transfection assay showed that the natural polyelectrolyte-based nonviral complex incorporated multilayer not only had good cytocompatibility, but also possessed the in vitro gene transfection ability. This kind of surface-mediated nonviral complex incorporated multilayer may have great potential in the localized and controlled delivery of DNA in biomedical implants and tissue engineering application. (c) 2009 Elsevier B.V. All rights reserved.
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