Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 70, Issue 1, Pages 91-97Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2008.12.016
Keywords
Atom transfer radical polymerization; Lectin; Localized surface plasmon resonance; Molecular recognition; Polymer brush; Sugar
Funding
- Japan Society for the Promotion of Science, Toyoko [19350055]
- Ministry of Education, Culture, Sports, Science and Technology, Tokyo [20106007]
- Grants-in-Aid for Scientific Research [19350055] Funding Source: KAKEN
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A disulfide-carrying polymer with many pendent mannose residues (dithiolated poly(2-methacryloyloxyethyl D-mannopyranoside)) was obtained by atom transfer radical polymerization (ATRP) method using 2-(2'-bromoisobutyroyloxy)ethyl disulfide as initiator. The obtained disulfide-carrying glycopolymer (DT-PMEMan) was accumulated as a polymer brush on a colloidal gold-immobilized glass substrate, and the binding processes of a lectin, Concanavalin A (Con A), to mannose residues in the polymer brush were examined using a UV-vis spectrophotometer with the help of a localized surface plasmon resonance. Con A showed a concentration-dependent specific binding to the glycopolymer brush, and the apparent association constant of Con A with mannose residues in the brush was much larger than the association constant for small sugars due to the so-called cluster effect. Furthermore, the glycopolymer brush-coated device had a detection limit lower than 5 nM. The glycopolymer-carrying device examined here is expected to expand our knowledge of recognition phenomena at the surface of polymer brushes. (C) 2008 Elsevier B.V. All rights reserved.
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