Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 66, Issue 2, Pages 213-217Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2008.06.020
Keywords
polydiacetylene; dimyristoyl phosphatidylcholine (DMPC); FTIR; label-free biosensor; bacteria sensor
Funding
- Korea Research Foundation [KRF-2006-005-J03603]
- Ministry of Health and Welfare [MOH-A050750]
- Korea Science and Engineering Foundation (Protein Chip Technology program)
- Korea Foundation for International Cooperation of Science Technology
- Brain Korea 21 Project
- Ministry of Education, Science & Technology (MoST), Republic of Korea [2007-00476] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2006-005-J03603, 2006-08359, 과C6A1901] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Micro-arrayed polydiacetylene (PDA) vesicles mixed with phospholipids on glass slides were prepared for label-free detection of Escherichia coli. When E. coli bound to its antibodies chemically attached to polydiacetylene, the fluorescence of the vesicles was dramatically increased. The insertion of dimyristoyl phosphatidylcholine (DMPC) in the vesicles drastically reduced the response time for the fluorescence changes. Vesicles with 20-30% DMPC provided optimal results for bacterial detection. Fourier transform infrared (FTIR) spectra analysis Suggested that DMPC insertion decreased the strength of hydrogen bonding among the amide and carboxylic acid groups of the polydiacetylene vesicles. Reduced bonding strength resulted in less rigid structure of the polydiacetylene polymer, allowing more rapid detection upon molecular recognition. (C) 2008 Elsevier B.V. All rights reserved.
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