Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 66, Issue 1, Pages 125-133Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfb.2008.06.006
Keywords
cell adhesion; cell culture; microsphere; particle; particle monolayer; surface topography; surface roughness; cell sheet
Funding
- High-Tech Research Center
- MEXT
- JSPS [19300176]
- Grants-in-Aid for Scientific Research [19300176] Funding Source: KAKEN
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We studied the topographical effect of roughness displayed by a closely packed particle monolayer on formation of a cell monolayer(cell sheet). Particle monolayers were prepared by Langmuir-Blodgett deposition using particles, which were 527 run (SA053) and 1270 nm (SA127) in diameter. Human umbilical vein endothelial cells (HUVECs) were seeded at a high density (2.0 x 10(5) cells/cm(2)) onto particle monolayers. It was found that cells gradually became into contact with adjacent cells on the SA053 monolayer and the formed cell sheet could be readily detached from the particle monolayer by gentle pipetting. On the other hand, cells adhering onto the tissue culture polystyrene (TCPS) and the SA127 particle monolayer were difficult to peel off. At a low cell seeding density (5.0 x 10(4) cells/cm(2)), pre-coating with bovine plasma fibronectin (FN) allowed cell growth on an SA053 particle monolayer. and a confluent monolayer was able to be peeled as a cell sheet from the particle monolayer just by pipetting. By immunostaining of human fibronectin, we found that fibronectin was secreted and concentrated onto the substrate side of a cell sheet. The obtained cell sheet adhered and grew on the TCPS again within 20 min. (c) 2008 Elsevier B.V. All rights reserved.
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