Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 62, Issue 1, Pages 11-16Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2007.09.006
Keywords
collagen; UV radiation; circular dichroism (CD); viscosity; fluorescence; absorption
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Research on the effect of UV radiation on stabilized collagen is an area of potential interest owing to the fact that collagen is an important biomaterial finding immense use in various fields. In this present study, effect of UV irradiation on collagen stabilized using chromium(III) has been studied. The physical and optical properties affected by UV irradiation have been detailed. Viscosity measurements have shown that chromium(III) treated collagen has better stability against UV radiation than native collagen. Circular dichroic studies indicate that increase in concentration of chromium(III) does not affect the conformation of collagen however, the duration of irradiation has profound impact on the conformation of collagen. The fluorescence intensity of native collagen has been found to decrease more than that of chromium(III) treated collagen. The difference absorption spectra also shows that chromium(III) treatment brings about more stability to collagen against UV irradiation. (c) 2007 Elsevier B.V. All rights reserved.
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