Journal
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
Volume 340, Issue 1-3, Pages 155-160Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.colsurfa.2009.03.023
Keywords
Escherichia coli (E. coli); Inclusion bodies (IBs); Electrophoretic deposition (EPD); Separation
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Funding
- Slovenian Research Agency
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This paper shows, for the first time, that the electrophoretic deposition technique is able to selectively collect protein inclusion bodies (PBs) from the host bacteria Suspensions. In the first step, zeta potential as a function of pH is carefully determined for both species involved. Based oil the obtained dependencies, the pH of the mixture of PBs and bacteria is precisely adjusted and the electrophoretic experiment is carried out. We show that the efficiency of separation and the yield depends not only on the electrokinetic properties of given species but also oil the electrode composition and Surface morphology. The deposited species are easily removed by forced washing or reverse electric field. As a whole, the selectivity and the yields are higher than in most alternative state-of-the art techniques. (C) 2009 Elsevier B.V. All rights reserved.
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