Electrochemiluminescent detection of hydrogen peroxide using amphiphilic luminol derivatives in solution

Amphiphilic luminol derivatives have been evaluated for electrochemiluminescence (ECL) measurements with a view to develop new lipid membranes as sensing element of nanobiosensors. The design and synthesis of new biospecific luminol derivatives with various substitution groups have been then undertaken. In a first approach, the possibility to use polarized screen-printed electrodes to trigger ECL in a buffer solution containing luminol derivatives and hydrogen peroxide has been studied. The ECL response to H2O2 in the presence of these luminescent derivatives has been investigated taking into account crucial factors such as the applied potential value, concentration of luminol derivatives and the substitution group in molecular structures. The lower hydrogen peroxide concentration detected varied from 1 X 10(-7) M to 5 x 10(-5) M, depending on the substitution group in the luminol derivative structures. (c) 2008 Elsevier B.V. All rights reserved.