Journal
ACS NANO
Volume 9, Issue 10, Pages 9922-9929Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.5b03413
Keywords
G-quadruplex; i-motif; single-molecule observation; DNA origami; high-speed AFM
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Funding
- Core Research for Evolutional Science and Technology (CREST) of JST
- JSPS KAKENHI [15H03837, 24104002, 24225005, 26620133]
- Sekisui Chemical Research Grant
- Kurata Memorial Hitachi Science and Technology Foundation
- National Science Foundation of China [21432008]
- Grants-in-Aid for Scientific Research [24104002, 26620133, 15H03837] Funding Source: KAKEN
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We demonstrate the single-molecule operation and observation of the formation and resolution of double-stranded DNA (dsDNA) containing a G-quadruplex (GQ) forming and counterpart i-motif forming sequence in the DNA nanostructure. Sequential manipulation of DNA strands in the DNA frame was performed to prepare a topologically controlled GQ/i-motif dsDNA. Using strand displacement and the addition and removal of K+, the topologically controlled GQ/i-motif dsDNA in the DNA frame was obtained in high yield. The dsDNA was resolved into the single-stranded DNA, GQ, and i-motif by the addition of K-F and operation in acidic conditions. The dissociation of the dsDNA under the GQ and i-motif formation condition was monitored by high-speed atomic force microscopy. The results indicate that the dsDNA containing the GQ- and i-motif sequence is effectively dissolved when the duplex is helically loosened in the DNA nanoscaffold.
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