Journal
COLLOID AND POLYMER SCIENCE
Volume 288, Issue 10-11, Pages 1113-1119Publisher
SPRINGER
DOI: 10.1007/s00396-010-2235-5
Keywords
Encapsulation; Emulsion electrospinning; Proteinase K; Poly(ethylene glycol)-poly(L-lactide)
Categories
Funding
- Natural Science Foundation of China [30828008]
- Natural Science Foundation of Tianjin, China [09JCZDJC18600]
Ask authors/readers for more resources
The aims of this study were to encapsulate water-soluble bioactive agents into biodegradable hydrophobic polymers via emulsion electrospinning for drug delivery and tissue engineering applications and propose a simple and facile method to evaluate the bioactivity of the encapsulated protein. Proteinase K was selected as a model protein to be incorporated into poly(ethylene glycol)-poly(l-lactide) (PELA) ultrafine fibers. Core-shell structured fibers with single core or multi-core were observed. In vitro release study showed that after a burst release at the early stage, a sustained release was achieved, indicating that proteinase K was incorporated inside ultrathin fibers successfully. Results of in vitro incubation in Tris-HCl buffer at pH 8.6 and 37 A degrees C revealed that electrospun PELA membranes containing proteinase K (PELA-P) showed obvious morphological changes, large mass loss, and slight decreases in melting temperature, melting enthalpy and relative molecular mass in 7 days. Additionally, a significant drop in pH value of the buffer after incubation of the PELA-P membrane was also observed. These findings clearly showed that encapsulation of water-soluble bioactive agents inside hydrophobic polymers could be achieved by emulsion electrospinning without compromising their bioactivity.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available