Journal
CNS NEUROSCIENCE & THERAPEUTICS
Volume 19, Issue 12, Pages 954-962Publisher
WILEY
DOI: 10.1111/cns.12195
Keywords
Action potential; Ion channel; Patch-clamp technique; Postnatal development; Visceral afferent
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Funding
- Chinese Natural Science Foundation [30973532, 81173051, 31171122]
- Education Department [20112307110008]
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BackgroundBaroreflex gain increase up closely to adult level during initial postnatal weeks, and any interruption within this period will increase the risk of cardiovascular problems in later of life span. We hypothesize that this short period after birth might be critical for postnatal development of vagal ganglion neurons (VGNs). MethodsTo evaluate neuroexcitability evidenced by discharge profiles and coordinate changes, ion currents were collected from identified A- and C-type VGNs at different developmental stages using whole-cell patch clamping. ResultsC-type VGNs underwent significant age-dependent transition from single action potential (AP) to repetitive discharge. The coordinate changes between TTX-S and TTX-R Na+ currents were also confirmed and well simulated by computer modeling. Although 4-AP or iberiotoxin age dependently increased firing frequency, AP duration was prolonged in an opposite fashion, which paralleled well with postnatal changes in 4-AP- and iberiotoxin-sensitive K+ current activity, whereas less developmental changes were verified in A-types. ConclusionThese data demonstrate for the first time that the neuroexcitability of C-type VGNs increases significantly compared with A-types within initial postnatal weeks evidenced by AP discharge profiles and coordinate ion channel changes, which explain, at least in part, that initial postnatal weeks may be crucial for ontogenesis in visceral afferent reflex function.
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