Effects of topical applications of porcine acellular urinary bladder matrix and Centella asiatica extract on oral wound healing in a rat model

ObjectivesTo evaluate the effects of topical applications of porcine acellular urinary bladder matrix (AUBM) and Centella asiatica extract (CAE) on the healing of tongue wounds in a rat model.Materials and methodsWounds were made in the tongue using a punch tool in 64 male Sprague-Dawley rats, randomized into four groups (n=16 per group): group 1 (control), group 2 (CAE), group 3 (AUBM mixed with orabase), and group 4 (orabase). No product was applied in group 1 and groups 2-4 received three daily topical applications. The animals were weighed on day 0 and at the time of euthanasia. Four rats in each group were euthanized at days 2, 7, 14, and 21 and the tongues were processed for: macroscopic morphometric analysis, myeloperoxidase (MPO) and malondialdehyde (MDA) levels, histological wound repair (degree of reepithelialization and inflammation), and CD31 positivity.ResultsThe animals' weight gain, histological wound repair, and CD31 positivity from greatest to least were: AUBM>CAE>orabase>control. Percentage of tongue occupied by wound, MPO, and MPA levels from least to greatest were: AUBM