Journal
CLINICAL ORAL INVESTIGATIONS
Volume 19, Issue 1, Pages 97-107Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00784-014-1224-3
Keywords
Essential oil; Chlorhexidine; Mouthwash; Substantivity; Plaque-like biofilm; Laser scanning confocal microscopy
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Funding
- Carlos III Institute of Health (General Division of Evaluation and Research Promotion, Madrid, Spain) [PI11/01383]
- European Regional Development Fund (ERDF)
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This study aims to evaluate the in situ antibacterial activity of a mouthwash containing essential oils (M-EO) on undisturbed de novo plaque-like biofilm (PL-biofilm) up to 7 h after its application. An appliance was designed to hold six glass disks on the buccal sides of the lower teeth, allowing PL-biofilm growth. Fifteen healthy volunteers wore the appliance for 48 h and then performed a M-EO. Disks were removed after 30 s and at 1, 3, 5, and 7 h later. After a washout period, the same procedure was repeated with a M-WATER and a M-0.2 % chlorhexidine. After PL-biofilm vital staining, samples were analyzed using a confocal laser scanning microscope. At 30 s after M-EO, the levels of bacterial vitality were 1.18 %, significantly lower than that of the basal sample (p < 0.001). After 7 h, the antibacterial effect of essential oils was still patent with a 47.86 % difference in bacterial vitality compared to the basal sample (p < 0.001). A single M-EO presents high antibacterial immediate activity and penetration capacity in situ and a substantivity which lasts for at least 7 h after its application over de novo biofilm. These results were better than those observed with 0.2 % chlorhexidine under the same conditions. A single M-EO is an effective measure against the de novo biofilm, presenting a good alternative to clorhexidine such as a preoperative rinse, in periodontal procedures or post-treatment applications.
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