Journal
CLINICAL NUTRITION
Volume 30, Issue 6, Pages 845-851Publisher
CHURCHILL LIVINGSTONE
DOI: 10.1016/j.clnu.2011.05.005
Keywords
Probiotics; Immune modulation; CD4(+) T cells; Human; Regulatory T cells
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Funding
- Senter Novem, an agency of the Dutch Ministry of Economic Affairs [FND06015]
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Background & aims: Probiotic bacteria are used as food supplement in many different disease settings. The immune modulating capacity of different strains is not always properly tested which might result in a suboptimal choice of strains for clinical use. Methods: The CD4 T cell responses to 19 different gut derived lactic acid bacteria were tested with different methods to show their diversity in immune modulation and to make a well-founded choice on which strains to use in future clinical trials. After co-culture of PBMC with bacteria, the induction of CD4(+) T cell subsets (regulatory T cells, T helper type (TH)1, TH2 and TH17) was analysed by rtPCR of transcription factor mRNA, intracellular FACS staining of transcription factors and cytokine production. Results: Bacterial strains all have diverse, unique immune modulatory properties. Strains call induce Treg, TH1, TH2 and TH17 cells which can be shown at different levels of T cell activation, and is consistent for most strains tested. For TH1, TH17 and Treg, a positive correlation between the different methods was found. For TH2 cells the correlation was less consistent. Conclusions: Probiotic bacteria have very different immune modulating capacities. Analysis of transcription factor mRNA is a suitable method for in vitro characterization of strains prior to clinical application. (C) 2011 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.
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