Journal
CLINICAL MICROBIOLOGY AND INFECTION
Volume 19, Issue 6, Pages 551-557Publisher
ELSEVIER SCI LTD
DOI: 10.1111/j.1469-0691.2012.03926.x
Keywords
Adenovirus; genotype; oligonucleotide array-in-well; real-time PCR; up-converting phosphor
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Funding
- Graduate School of Advanced Diagnostic Technologies and Applications (DIA-NET)
- Finnish Funding Agency for Technology and Innovation (TEKES)
- Jenny and Antti Wihuri Foundation
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Clin Microbiol Infect Abstract A robust oligonucleotide array-in-well hybridization assay using novel up-converting phosphor reporter technology was applied for genotyping clinically relevant human adenovirus types. A total of 231 adenovirus-positive respiratory, ocular swab, stool and other specimens from 219 patients collected between April 2010 and April 2011 were included in the study. After a real-time PCR amplification targeting the adenovirus hexon gene, the array-in-well assay identified the presence of B03 (n=122; 57.5% of patients), E04 (29; 13.7%), C02 (21; 9.9%), D37 (14; 6.6%), C01 (12; 5.7%), C05 (5; 2.4%), D19 (4; 1.9%), C06 (2; 0.9%), D08 (1; 0.5%), A31 (1; 0.5%) and F41 (1; 0.5%) genotypes among the clinical sample panel. The typing result was obtained for all specimens that could be amplified (n=223; 97%), and specificity of the typing was confirmed by sequencing specimens representing each of the different genotypes. No hybridization signal was obtained in adenovirus-negative specimens or specimens with other viruses (n=30). The array-in-well hybridization assay has great potential as a rapid and multiplex platform for the typing of clinically relevant human adenovirus genotypes in different specimen types.
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