Journal
CLINICAL MICROBIOLOGY AND INFECTION
Volume 18, Issue 5, Pages 461-467Publisher
ELSEVIER SCI LTD
DOI: 10.1111/j.1469-0691.2011.03593.x
Keywords
Bovine mastitis; main spectra library; MALDI Biotyper; microflex; Prototheca
Categories
Funding
- Deutsche Forschungsgemeinschaft (DFG) [RO 2518/3-1]
Ask authors/readers for more resources
The possibility of using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF similar to MS) for rapid identification of pathogenic and non-pathogenic species of the genus Prototheca has been recently demonstrated. A unique reference database of MALDI-TOF similar to MS profiles for type and reference strains of the six generally accepted Prototheca species was established. The database quality was reinforced after the acquisition of 27 spectra for selected Prototheca strains, with three biological and technical replicates for each of 18 type and reference strains of Prototheca and four strains of Chlorella. This provides reproducible and unique spectra covering a wide m/z range (200020 000 Da) for each of the strains used in the present study. The reproducibility of the spectra was further confirmed by employing composite correlation index calculation and main spectra library (MSP) dendrogram creation, available with MALDI Biotyper software. The MSP dendrograms obtained were comparable with the 18S rDNA sequence-based dendrograms. These reference spectra were successfully added to the Bruker database, and the efficiency of identification was evaluated by cross-reference-based and unknown Prototheca identification. It is proposed that the addition of further strains would reinforce the reference spectra library for rapid identification of Prototheca strains to the genus and species/genotype level.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available