Journal
CLINICAL MICROBIOLOGY AND INFECTION
Volume 18, Issue 1, Pages 81-85Publisher
WILEY-BLACKWELL
DOI: 10.1111/j.1469-0691.2011.03540.x
Keywords
ELISA; rk-28; serodiagnosis; visceral Leishmaniasis
Categories
Funding
- National Institute of Allergy and Infectious disease (NIAID), DMID
- Tropical Medicine Research Center [P50AI074321]
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Antibody detection is a safely applied method at the wide scale in diagnosis of visceral Leishmaniasis (VL). In order to further advance serodiagnosis, the rK28 antigen has been recently introduced as a candidate for diagnosis of VL. We evaluated the sensitivity and specificity of the rK28 antigen in a micro-ELISA format in comparison to the rk39 antigen. The test was conducted on 252 parasitologically confirmed VL cases, 103 endemic healthy controls, 95 non-endemic healthy controls, 88 other infectious disease and 53 follow-up cases. Of 252 parasitologically confirmed VL cases, 251 cases were reported positive by rK28 antigen, yielding 99.6% sensitivity (95% CI, 0.970.99), which was similar to the sensitivity of rK39 ELISA (99.6%) (95% CI, 0.970.99). Specificity of the rK28 antigen in non-endemic and endemic healthy controls was 100% (95% CI 0.961) and 94.17% (95% CI, 0.880.97), respectively. In 88 different diseases, specificity was 95.45% (95% CI, 0.840.96). With the rK39 antigen, specificity of non-endemic and endemic controls and different diseases was 100% (95% CI 0.961), 92.23% (95% CI 0.850.96) and 96.59% (95% CI 0.900.98), respectively. Our results show that rK39 and rK28 antigens have similar sensitivity and specificity and rK28 can also be used as a serodiagnostic tool in the endemic population of Bihar.
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