Journal
CLINICAL IMMUNOLOGY
Volume 139, Issue 2, Pages 122-132Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.clim.2011.01.002
Keywords
CD14(+)CD16(++) monocytes; Intravenous immunoglobulins; CD32B; Common variable immunodeficiency
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Funding
- National Committee for Scientific Research [3220/P01/2007/32]
- Alexander von Humboldt Foundation
- Deutsche Forschungsgemeinschaft [Zi288]
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We have studied the effect of intravenous immunoglobulins (IVIG) on monocyte subpopulations and cytokine production in patients with CVID. The absolute number of CD14(+)CD16(++) monocytes decreased on average 2.5-fold 4 h after IVIG and after 20 h returned to the baseline. The cytokine level in the supernatants of peripheral blood mononuclear cells (PBMC) after ex vivo LPS stimulation demonstrated the > 2-fold decrease in TNF production 4 h after IVIG. The TNF expression, which is higher in the CD14(+)CD16(++) monocytes, was decreased in these cells by IVIG in 4/7 CVID cases. In vitro exposure of the healthy individuals' monocytes to the IVIG preparation resulted in reduced TNF production, which was overcome by blockade of the Fc gamma RIIB in the CD14(+)CD16(++) CD32B(high) monocytes. Our data suggest that reduction in the number of CD14(+)CD16(++) monocytes and the blockade of their cytokine production via triggering CD32B can contribute to the anti-inflammatory action of IVIG. (C) 2011 Elsevier Inc. All rights reserved.
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