Journal
CLINICAL IMMUNOLOGY
Volume 138, Issue 1, Pages 77-84Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.clim.2010.09.009
Keywords
Enterohemorrhagic E. coli; E. coli secreted protein A (EspA); Epitope; Epitope mapping
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Funding
- National High-Tech Research and Development Program of China [2006AA02Z443, 2007AA02Z409]
- National Natural Science Foundation of China [30770514, 30670107]
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Enterohemorrhagic E. coli (EHEC) causes severe diseases in humans and animals via the production of Shiga toxins, and injection of effectors into epithelia using type III secretion system (TTSS). E. coli secreted protein A (EspA) forms the filamentous conduits of TTSS, which extends into the translocation pore embedded in host cell membranes and aids in the transportation of bacterial effectors. In addition, EspA is closely associated with initial bacterial adhesion and the formation of biofilms. EspA in its various forms elicits protective immune responses, although the epitope responsible has not to be identified. Here we report the presence of a linear, immunogenic, conserved and partially protective epitope E07 (100Lys-120Val) on EspA, which is recognized by the novel monoclonal antibody 1H10. This antibody blocks EHEC-induced actin polymerization and confers protection in mice. These findings provide a better understanding of EspA-induced immune responses and could lead to epitope-based vaccines and antibody-based therapies. (C) 2010 Elsevier Inc. All rights reserved.
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