Journal
CLINICAL IMMUNOLOGY
Volume 132, Issue 3, Pages 362-370Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.clim.2009.05.011
Keywords
CD40 ligand; DNA methylation; Immunoglobulin G; Systemic lupus; erythematosus
Categories
Funding
- National Natural Science Foundation of China [30730083, 30671883]
- National Basic Research Program of China [2009CB825605]
- Hunan Natural Science Foundation [06C0049]
- Science Foundation of Hunan Province [06SK3033]
- NIH [P20-RR015577, R03AI076729]
- National Center for Research Resource
- Arthritis National Research Foundation
- University of Oklahoma College of Medicine (AHS)
- NATIONAL CENTER FOR RESEARCH RESOURCES [P20RR015577] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R03AI076729] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES [R01AR042525] Funding Source: NIH RePORTER
Ask authors/readers for more resources
CD40 ligand (CD40LG), encoded on the X chromosome, has been reported to be overexpressed on lupus T cells. Herein, we investigated the effect of DNA demethylation on T cell CD40LG expression and the production of IgG by autologous B cells in lupus. We found normal human T cells transfected with CD40LG induced autologous B cell activation and plasma cell differentiation. Both female lupus CD4+ T cells and demethylating agents treated CD4+ T cells overexpressed CD40LG mRNA. Further, lupus T cells from both genders or demethylated CD4+ T cells from healthy women overstimulated autologous B cells, and this could be reversed with anti-CD40LG Ab in only females. We demonstrated that female lupus CD4+ T cells and demethylated CD4+ T cells express high level of CD40LG and overstimulate B cells to produce IgG. This is due to DNA demethylation and thereby reactivation of the inactive X chromosome in female. (C) 2009 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available