Journal
CLINICAL IMMUNOLOGY
Volume 130, Issue 2, Pages 199-212Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.clim.2008.09.009
Keywords
Autoimmune thrombocytopenia; B cells; Splenectomy; Immunological memory
Categories
Funding
- DFG [Do491/7-1, Do491/5-5]
- [SFB650/TP16]
- [SFB421/B13]
- [Z3]
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The effect of splenectomy on circulating memory B cells in autoimmune thrombocytopenia purpura (AITP) patients has not yet been addressed. We therefore analyzed the distribution and phenotypic characteristics of B-cell. subsets in non-splenectomized and splenectomized AITP patients and controls, as well as CD95 expression after B cell. activation. Decreased frequencies of memory B cells in splenectomized individuals were observed, with a rapid decline of CD27+IgD+ and a slower decrease of CD27+IgD- and CD27-/IgD- cells. Similar results were noted following splenectomy in healthy donors (HD). CD95+ B cells were substantially increased in all subsets in patients with active AITP, indicating their enhanced activation status. After splenectomy, the percentage of CD95+ B cells were further increased in the CD27+IgD- post-switch memory population in AITP, but not in HD. CD95+CD27+ memory B cells largely reside in the region in the human spleen analogous to the murine marginal zone. Thus, the spleen plays a fundamental role in controlling peripheral memory B cell homeostasis in both AITP and HD and regulates activated CD95+ B cells in patients with AITP. (C) 2008 Elsevier Inc. All rights reserved.
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