Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 54, Issue 16, Pages 4890-4894Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201500537
Keywords
co-culture systems; fluorescence probes; hypochlorite generation; two-photon microscopy
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Funding
- National Creative Research Initiative programs of the National Research Foundation of Korea (NRF) - Korean government (MSIP) [2012R1A3A2048814]
- National Research Foundation of Korea (NRF) - Korean government (MSIP) [2012R1A2A1A03670456]
- National Research Foundation of Korea [2012R1A2A1A03670456] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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We designed and prepared the imidazoline-2-thione containing OCl- probes, PIS and NIS, which operate through specific reactions with OCl- that yield corresponding fluorescent imidazolium ions. Importantly, we demonstrated that PIS can be employed to image OCl- generation in macrophages in a co-culture system. We have also employed two-photon microscopy and PIS to image OCl- in live cells and tissues, indicating that this probe could have wide biological applications.
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