Journal
JOURNAL OF LUMINESCENCE
Volume 168, Issue -, Pages 62-68Publisher
ELSEVIER
DOI: 10.1016/j.jlumin.2015.07.030
Keywords
BSA Au nanoclusters; Tryptophan emission; Fluorescence; Quenching; Denaturants
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Funding
- NIH [R01EB12003]
- NSF [CBET-1264608]
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1264608] Funding Source: National Science Foundation
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In this paper, we have synthesized BSA protected gold nanoclusters (BSA Au nanocluster) and studied the effect of quencher, protein denaturant, pH and temperature on the fluorescence properties of the tryptophan molecule of the BSA Au nanocluster and native BSA. We have also studied their effect on the peak emission of BSA Au nanoclusters (650 nm). The photophysical characterization of a newly developed fluorophore in different environments is absolutely necessary to futher develop their biomedical and analytical applications. It was observed from our experiments that the tryptophan in BSA Au nanoclusters is better shielded from the polar environment Tryptophan in native BSA showed a red shift in its peak emission wavelength position. Tryptophan is a highly polarity sensitive dye and a minimal change in its microenvironment can be easily observed in its photophysical properties. (C) 2015 Elsevier B.V. All rights reserved.
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