Journal
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
Volume 47, Issue 3, Pages 305-310Publisher
WALTER DE GRUYTER GMBH
DOI: 10.1515/CCLM.2009.083
Keywords
biomarker; cirrhosis; gas chromatography/mass spectrometry; hepatitis B virus (HBV); metabolomic
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Funding
- National High Technology Research and Development Program of China 863 Project [2006AA02Z4C5]
- National Basic Research Program of China 973 program [2007CB936004]
- National Natural Science Foundation of China [30772505]
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Background: Metabolomic studies have been applied to disease biomarkers selection. With the metabolomic technique, gas chromatography/mass spectrometry (GC/MS), human serum metabolites can be detected and identified. The purpose of this study was to investigate the serum metabolic profile of hepatitis B virus (HBV) infected cirrhosis patients and to detect disease biomarkers. Methods: HBV infected non-cirrhosis male subjects (ns20) and HBV infected cirrhosis male patients (ns20) participated in this experiment. Serum metabolome was detected through chemical derivatization followed by GC/MS. The high- flux metabolomic data were analyzed by stepwise discriminant analysis. Results: Out of the 41 metabolites detected in serum, we selected metabolites, including acetic acid, sorbitol, D-lactic acid, hexanoic acid, 1-naphthalenamine, butanoic acid, phosphoric acid, D-glucitol, and glucose, which in combination with each other could segregate the two groups. The error count was 0% for the non-cirrhosis group and 25% for the cirrhosis group. Conclusions: This technique can be used to select biomarkers for hepatic cirrhosis.
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