Journal
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
Volume 38, Issue 17, Pages 1622-1628Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10826076.2015.1087026
Keywords
amantadine; fluorescence derivatization; HPLC; microdialysis
Funding
- National Science Foundation of China [21275089, 81303179]
- Natural Science Foundation of Shandong Province [ZR2014BQ009]
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A new and sensitive high-performance liquid chromatography (HPLC) method with 10-methyl-acridone-2-sulfonyl chloride (MASC) as pre-column fluorescence labeling reagent was developed for the determination of amantadine in microdialysis samples from rat plasma. The HPLC sensitivity of amantadine was greatly enhanced by introducing MASC with large conjugated structure and excellent fluorescence property into the amantadine molecule. The limit of detection was 6.8ngmL(-1) and only 30L sample was needed for the pharmacokinetic study. The linearity range was 25-500ngmL(-1) with correlation coefficients of 0.9989. The proposed method was successfully applied to the determination of amantadine in microdialysis samples from rat plasma. It also shows powerful potential for the pharmacokinetic study of compounds with amino group.
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